Imp/OstA is required for cell envelope biogenesis in Escherichia coli
- 2 September 2002
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 45 (5) , 1289-1302
Abstract
Summary: In Gram‐negative bacteria, all components of the outer membrane are synthesized in the cytoplasm or the cytoplasmic leaflet of the inner membrane and must thus traverse the inner membrane and the periplasm on the way to their final destination. In this study, we show Imp/OstA to have characteristics typical for proteins involved in envelope biogenesis. Imp is essential and forms a high‐molecular‐weight disulphide‐bonded complex in the outer membrane. Upon depletion of Imp, lipids and outer membrane proteins appear in a novel membrane fraction with higher density than the outer membrane. We propose Imp to be part of a targeting/usher system for components of the outer membrane.Keywords
This publication has 33 references indexed in Scilit:
- Genetic Evidence for Parallel Pathways of Chaperone Activity in the Periplasm of Escherichia coliJournal of Bacteriology, 2001
- Characterization of the Escherichia coliςE RegulonJournal of Biological Chemistry, 2001
- An Escherichia coli Mutant Defective in Lipid ExportJournal of Biological Chemistry, 2001
- Evolutionarily Related Insertion Pathways of Bacterial, Mitochondrial, and Thylakoid Membrane ProteinsAnnual Review of Cell and Developmental Biology, 2000
- TARGETING AND ASSEMBLY OF PERIPLASMIC AND OUTER-MEMBRANE PROTEINS IN ESCHERICHIA COLIAnnual Review of Genetics, 1998
- Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K‐12Electrophoresis, 1997
- Mapping of Organic Solvent Tolerance GeneostAinEscherichia coliK-12Bioscience, Biotechnology, and Biochemistry, 1994
- Prediction of membrane‐spanning β‐strands and its application to maltoporinProtein Science, 1993
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesJournal of Molecular Biology, 1986
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983