Pyrimidine-specific chemical reactions useful for DNA sequencing
Authors:
- Carol M. RubinCRCarol M. Rubin
- Carl W. SchmidCSCarl W. Schmid
- 1 January 1980
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (20) , 4613-4620
Abstract
Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).Keywords
This publication has 4 references indexed in Scilit:
- Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequencesNature, 1980
- [57] Sequencing end-labeled DNA with base-specific chemical cleavages1980
- Base-specific reactions useful for DNA sequencing: methylene blue – sensitized photooxidation of guanine and osmium tetraoxide modification of thymineNucleic Acids Research, 1978
- The selective degradation of pyrimidines in nucleic acids by permanganate oxidationBiochemical and Biophysical Research Communications, 1967