PET-PCR method for the molecular detection of malaria parasites in a national malaria surveillance study in Haiti, 2011
Authors:
- Naomi W LucchiNLNaomi W Lucchi
- Mara A KarellMKMara A Karell
- Ito JournelIJIto Journel
- Eric RogierEREric Rogier
- Ira GoldmanIGIra Goldman
- Dragan LjoljeDLDragan Ljolje
- Curtis HuberCHCurtis Huber
- Kimberly E MaceKMKimberly E Mace
- Samuel E JeanSJSamuel E Jean
- Eniko E AkomEAEniko E Akom
- Roland OscarRORoland Oscar
- Josiane ButeauJBJosiane Buteau
Open Access
- 26 November 2014
- journal article
- Published by Springer Nature in Malaria Journal
- Vol. 13 (1) , 462
Abstract
Recently, a real-time PCR assay known as photo-induced electron transfer (PET)-PCR which relies on self-quenching primers for the detection of Plasmodium spp. and Plasmodium falciparum was described. PET-PCR assay was found to be robust, and easier to use when compared to currently available real-time PCR methods. The potential of PET-PCR for molecular detection of malaria parasites in a nationwide malaria community survey in Haiti was investigated.Keywords
This publication has 19 references indexed in Scilit:
- Asymptomatic malaria infections: detectability, transmissibility and public health relevanceNature Reviews Microbiology, 2014
- High prevalence of asymptomatic malaria in south-eastern BangladeshMalaria Journal, 2014
- Epidemiology of subpatent Plasmodium falciparum infection: implications for detection of hotspots with imperfect diagnosticsMalaria Journal, 2013
- The silent threat: asymptomatic parasitemia and malaria transmissionExpert Review of Anti-infective Therapy, 2013
- Association of sub-microscopic malaria parasite carriage with transmission intensity in north-eastern TanzaniaMalaria Journal, 2011
- Malaria prevalence defined by microscopy, antigen detection, DNA amplification and total nucleic acid amplification in a malaria‐endemic region during the peak malaria transmission seasonTropical Medicine & International Health, 2011
- A Research Agenda for Malaria Eradication: Diagnoses and DiagnosticsPLOS Medicine, 2011
- A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination settingMalaria Journal, 2010
- Molecular diagnostic and surveillance tools for global malaria controlTravel Medicine and Infectious Disease, 2008
- Is molecular biology the best alternative for diagnosis of malaria to microscopy? A comparison between microscopy, antigen detection and molecular tests in rural Kenya and urban TanzaniaTropical Medicine & International Health, 2006