Production of Functional Single-Chain Fv Antibodies in the Cytoplasm of Escherichia coli
- 1 June 2002
- journal article
- Published by Elsevier in Journal of Molecular Biology
- Vol. 320 (1) , 1-10
Abstract
No abstract availableKeywords
Funding Information
- Comunidad de Madrid
- European Commission (QLK3-CT1999-00041, QLK3-CT2000-00170)
- Ministerio de Educación, Cultura y Deporte
- Comisión Interministerial de Ciencia y Tecnología (BIO2001-2274)
- National Institutes of Health (GM55090)
This publication has 49 references indexed in Scilit:
- High level production of functional antibody fab fragments in an oxidizing bacterial cytoplasm11Edited by J. KarnJournal of Molecular Biology, 2002
- Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies 1 1Edited by R. HuberJournal of Molecular Biology, 2001
- Stability engineering of antibody single-chain Fv fragmentsJournal of Molecular Biology, 2001
- Importance of Redox Potential for the in Vivo Function of the Cytoplasmic Disulfide Reductant Thioredoxin from Escherichia coliJournal of Biological Chemistry, 1999
- Expression of an antibody fragment at high levels in the bacterial cytoplasmJournal of Molecular Biology, 1998
- Antibody scFv fragments without disulfide bonds, made by molecular evolutionJournal of Molecular Biology, 1998
- A natural antibody missing a cysteine in VH: consequences for thermodynamic stability and foldingJournal of Molecular Biology, 1997
- Refolding of bovine pancreatic trypsin inhibitor via non-native disulphide intermediatesJournal of Molecular Biology, 1995
- Identification of a protein required for disulfide bond formation in vivoCell, 1991
- Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coliGene, 1988