Gene Expression inPorphyromonas gingivalisafter Contact with Human Epithelial Cells
Open Access
- 1 April 2005
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 73 (4) , 2327-2335
Abstract
Porphyromonas gingivalis, a gram-negative oral anaerobe, is strongly associated with adult periodontitis. The adherence of the organism to host epithelium signals changes in both cell types as bacteria initiate infection and colonization and epithelial cells rally their defenses. We hypothesized that the expression of a defined set ofP. gingivalisgenes would be consistently up-regulated during infection of HEp-2 human epithelial cells.P. gingivalisgenome microarrays were used to compare the gene expression profiles of bacteria that adhered to HEp-2 cells and bacteria that were incubated alone. Genes whose expression was temporally up-regulated included those involved in the oxidative stress response and those encoding heat shock proteins that are essential to maintaining cell viability under adverse conditions. The results suggest that contact with epithelial cells induces inP. gingivalisstress-responsive pathways that promote the survival of the bacterium.Keywords
This publication has 50 references indexed in Scilit:
- Comparative Whole-Genome Analysis of Virulent and Avirulent Strains ofPorphyromonas gingivalisJournal of Bacteriology, 2004
- Studies on NADH oxidase and alkyl hydroperoxide reductase produced by Porphyromonas gingivalisOral Microbiology and Immunology, 2004
- Pathways of Oxidative DamageAnnual Review of Microbiology, 2003
- Identification and Testing of Porphyromonas gingivalis Virulence Genes with a pPGIVET SystemInfection and Immunity, 2002
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Quantification of Bacterial Transcripts during Infection Using Competitive Reverse Transcription-PCR (RT-PCR) and LightCycler RT-PCRClinical and Diagnostic Laboratory Immunology, 2001
- Roles of the Glutathione- and Thioredoxin-Dependent Reduction Systems in the Escherichia Coli and Saccharomyces Cerevisiae Responses to Oxidative StressAnnual Review of Microbiology, 2000
- Characterization of the BatI (Bacteroides aerotolerance) operon in Bacteroides fragilis: isolation of a B. fragilis mutant with reduced aerotolerance and impaired growth in in vivo model systemsMolecular Microbiology, 1999
- Basic local alignment search toolJournal of Molecular Biology, 1990
- A tissue culture model for study of growth promotion and antimicrobial susceptibifity in Bacteroides fragilisJournal of Antimicrobial Chemotherapy, 1987