Isolation of Murine Hematopoietic Stem Cells and Progenitor Cells
Open Access
- 1 June 2005
- journal article
- unit
- Published by Wiley in Current Protocols in Immunology
- Vol. 67 (1) , 22B.1.1-22B.1.13
Abstract
Protocols for the purification of CD34−KSL cells, CD34+KSL cells, SP cells, and Tip-SP CD34−KSL cells are described. Mouse hematopoietic stem cells are highly enriched in the CD34−KSL and SP fractions. These two populations overlap one another. A nearly homogenous population of HSCs can be obtained by isolating Tip-SP CD34−KSL cells. One of the earliest progenitor cell populations is CD34+KSL cells.Keywords
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