Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins.
Authors:
Open Access
- 1 December 1993
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 7 (12a) , 2378-2391
Abstract
A two-hybrid protein interaction screen was used to isolate cDNAs encoding human proteins that can interact with human CDK2 in yeast. A new member of the retinoblastoma susceptibility gene family, Rbr-2 (Rb-related), was obtained. The sequence of the Rbr-2 protein shares approximately 50% identify with p107 and homology to Rb within the pocket domain. Several lines of evidence indicate that Rbr-2 is the adenovirus E1A-associated p130. Like Rb and p107, p130Rbr-2 can bind to viral oncoproteins, SV40 large T antigen, and adenovirus E1A through its pocket domain. Although p130Rbr-2 does not bind to CDK2 in vitro, it can interact with cyclins, with a clear preference for D-type cyclins. Because both CDK2 and p130Rbr-2 show affinity for cyclins, we suggest that p130Rbr-2 and CDK2 interacted through a yeast-derived cyclin bridge in the two-hybrid screen. The gene encoding p130Rbr-2 mapped to 16q13, a region of frequent genomic alteration in human tumors.Keywords
This publication has 46 references indexed in Scilit:
- Chromosome Mapping of Human CDC25A and CDC25B PhosphatasesGenomics, 1993
- A‐ and B‐type cyclins differentially modulate substrate specificity of cyclin‐cdk complexes.The EMBO Journal, 1993
- Molecular cloning, chromosomal mapping, and expression of the cDNA for p107, a retinoblastoma gene product-related proteinCell, 1991
- Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin ACell, 1991
- 1P13 is the most frequently involved band in structural chromosomal rearrangements in human breast cancerGenes, Chromosomes and Cancer, 1990
- Transcription of a nematode trans‐spliced leader RNA requires internal elements for both initiation and 3′ end‐formation.The EMBO Journal, 1990
- Two distinct and frequently mutated regions of retinoblastoma protein are required for binding to SV40 T antigen.The EMBO Journal, 1990
- The regions of the retinoblastoma protein needed for binding to adenovirus E1A or SV40 large T antigen are common sites for mutations.The EMBO Journal, 1990
- An essential G1 function for cyclin-like proteins in yeastCell, 1989
- p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase.The EMBO Journal, 1987