The Sequence Determination of a Protein in a Micro Scale: The Sequence Analysis of Ribosomal Protein L34 ofEscherichia coli
Author:
- Robert ChenRCRobert Chen
- 1 January 1976
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 357 (1) , 873-886
Abstract
The complete amino acid sequence of ribosomal protein L34 has been established by improved micro techniques with 3 mg of the lyophilized protein. The protein was digested with trypsin, thermolysin and chymotrypsin and the resulting peptides were isolated from fingerprints performed on cellulose thin-layer plates. The amino acid sequences of the peptides were determined by the combined micro dansyl-Edman technique using 5 - 10 nmol per sample. Aspartic acid and glutamic acid were distinguished from their amides by use of the color reaction of ninhydrin with the respective amino acid phenylthiohydantoins.Keywords
This publication has 9 references indexed in Scilit:
- Studies on the primary structure of 14 proteins from the large subunit of escherichia coli ribosomes with an improved protein sequenator and with mass spectrometryJournal of Supramolecular Structure, 1975
- Staphylococcal Protease: A Proteolytic Enzyme Specific for Glutamoyl BondsProceedings of the National Academy of Sciences, 1972
- Chemical studies on methionyl-tRNA synthetase from Escherichia coliJournal of Molecular Biology, 1970
- A NEW METHOD FOR FRACTIONATION OF PROTAMINES AND THE AMINO ACID SEQUENCES OF SALMINE AND THREE COMPONENTS OF IRIDINEInternational Journal of Protein Research, 1969
- High recovery of tryptophan from acid hydrolysates of proteinsBiochemical and Biophysical Research Communications, 1969
- Révélation colorée des spots de phénylthiohydantoi̋ne d'acides aminésJournal of Chromatography A, 1969
- Amino Acid Sequence of the f2 Coat ProteinJournal of Biological Chemistry, 1967
- The Preparation and Enzymatic Hydrolysis of Reduced and S-Carboxymethylated ProteinsJournal of Biological Chemistry, 1963
- SELECTIVE CLEAVAGE OF THE METHIONYL PEPTIDE BONDS IN RIBONUCLEASE WITH CYANOGEN BROMIDE1Journal of the American Chemical Society, 1961