Identification of HR-Inducing cDNAs from Plant Pathogens via a Gateway®-Compatible Binary Potato Virus X-Expression Vector
- 23 November 2011
- book chapter
- Published by Springer Nature
- Vol. 835, 97-105
Abstract
Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a cDNA library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing cDNAs from plant pathogens.Keywords
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