Avian Influenza Virus Isolation, Propagation, and Titration in Embryonated Chicken Eggs
Authors:
- 17 May 2014
- book chapter
- Published by Springer Nature
- Vol. 1161, 125-140
Abstract
Avian influenza virus and some mammalian influenza A viruses are usually isolated, propagated, and titrated in embryonated chicken eggs (ECE). Most any sample type can be accommodated for culture with appropriate processing. Isolation may also be accomplished in cell culture particularly if mammalian lineage isolates are suspected, for example, swine influenza in turkey specimens. Culture is highly sensitive, but is not specific for influenza A, which may be an advantage because a sample may be screened for several agents at once. Once an agent is isolated in culture, the presence of influenza viruses is confirmed with any of several assays. The methods for propagating virus isolates in ECE are described.Keywords
This publication has 6 references indexed in Scilit:
- Optimal specimen collection and transport methods for the detection of avian influenza virus and Newcastle disease virusBMC Veterinary Research, 2013
- Evaluation of different embryonating bird eggs and cell cultures for isolation efficiency of avian Influenza A Virus and Avian paramyxovirus serotype 1 from real-time reverse transcription polymerase chain reaction–positive wild bird surveillance samplesJournal of Veterinary Diagnostic Investigation, 2012
- Single and Combination Diagnostic Test Efficiency and Cost Analysis for Detection and Isolation of Avian Influenza Virus from Wild Bird Cloacal SwabsAvian Diseases, 2010
- Evaluation and Attempted Optimization of Avian Embryos and Cell Culture Methods for Efficient Isolation and Propagation of Low Pathogenicity Avian Influenza VirusesAvian Diseases, 2010
- Low-Pathogenicity Avian Influenza Virus (H6N2) in Chickens in California, 2000–02Avian Diseases, 2003
- The Effect of Various Disinfectants on Detection of Avian Influenza Virus by Real Time RT-PCRAvian Diseases, 2003