Screening of the drought tolerant phosphate solubilizing bacteria in dissolving P-inorganic

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Abstract
The drought tolerant phosphate solubilizing bacteria is needed to dissolve inorganic phosphate (P) which is usually low in the availability at dry land. This study is aimed to obtain a combination of drought tolerant phosphate solubilizing bacteria which has high potential in dissolving P-inorganic. An experimental study which has 4 treatments of bacterial combinations has been conducted in a laboratory. The first treatment was the combination between Pseudomonas azotoformans (A) and Acinetobacter baumannii (B). The second treatment was the combination of A and Bacillus paramycoides (C). The third treatment was B and C, then the fourth treatment was A, B, and C. The potential of the bacterial combination in dissolving P-inorganic was measured qualitatively using phosphate solubilizing index (PSI) on pikovskaya solid medium. While, the potential of the bacterial combination in dissolving P-inorganic was measured quantitatively by measuring dissolved P using the ascorbic acid method in pikovskaya liquid medium containing 0.5% Ca3(PO4)2. The results showed that the combination of those three bacteria (A B and C) has the best potential to dissolve P-inorganic compared to other combinations. In addition, the three bacteria combination also resulted in the highest dissolved P with 0.29% potential dissolution of the total Ca3(PO4)2 contained in the pikovskaya liquid medium, followed by combination of B and C (0.19%), and A and C (0.18%), respectively. Thus, before the combination of these three bacteria is applied in soil, it is needed further experiment of the potential of the bacteria in dissolving soil P-inorganic.

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