Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry.
Authors:
- M M ReyndersMRM M Reynders
- F T BosmanFBF T Bosman
- G H BlijhamGBG H Blijham
Open Access
- 1 November 1987
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 35 (11) , 1343-1345
Abstract
We investigated the influence of various fixatives on monoclonal anti-BrdUrd antibody binding of BrdUrd-substituted DNA in tissue sections of routinely processed mouse small intestine after in vivo administration of BrdUrd. For denaturing fixatives such as ethanol or Carnoy's fluid, a standard denaturation protocol showed specific crypt cell labeling. With cross-linking agents such as formalin and glutaraldehyde, a remarkable increase in staining intensity was obtained after tissue digestion with pepsin before acid denaturation. The optimal pepsin concentration was determined for maximal immunoreactivity combined with acceptable morphology.Keywords
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