Runx2 Regulated Airway Homeostasis Is Disrupted in Asthma

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Abstract
In asthma, augmented airway wall smooth muscle (ASM) bulk is a major remodeling feature, promoted by increased transforming growth factor (TGF)‐β1 and connective tissue growth factor (CTGF). Runt‐related transcription factor‐2 (RUNX2) represses TGF‐β1‐induced CTGF through interactions with SMAD3. This study aimed to investigate the expression and role of RUNX2 in asthmatic and nonasthmatic ASM cells. mRNA and protein were detected by microarray, PCR, and western blot in nonasthmatic and asthmatic ASM cells. Immunohistochemistry identified RUNX2 in lung tissues from asthmatic patients and nonasthmatic subjects. Different RUNX2 isoforms were transfected into immortalized‐asthmatic ASM cells, and markers of inflammation and airway remodeling were measured. RUNX2 alternatively spliced forms were examined in bronchial biopsies from asthmatic and healthy subjects. The abundance of RUNX2 was decreased in isolated ASM cells from asthmatic compared with nonasthmatic subjects. The ASM layer around airways in lung tissue sections from asthmatic and nonasthmatic patients had a heterogeneous pattern of RUNX2 protein detection. TGF‐β1 stimulation increased RUNX2/RUNX2 variant 1 mRNA in nonasthmatic but not asthmatic ASM cells, facilitating SMAD3 activation and nuclear translocation in asthmatic ASM cells. RUNX2 isoform overexpression in immortalized asthmatic ASM cells failed to alter markers of inflammation (IL‐6) but significantly reduced markers of remodeling (CTGF), ASM cell hypertrophy (GSK‐3β and desmin), and proliferation (pSer 795 Rb and α‐tubulin). In bronchial biopsies, RUNX2 mRNA splicing was higher in asthmatic patients compared with healthy subjects. These data suggest RUNX2 plays a role in the homeostasis of healthy airways. Restoring RUNX2 may provide a new therapeutic approach for asthma.
Funding Information
  • National Health and Medical Research Council (#1061712)
  • National Health and Medical Research Council (#1032695)
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek (Aspasia 015.013.010)
  • National Natural Science Foundation of China (82000025)

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