A System for the Translation of Receptor Messenger-RNA and the Study of the Assembly of Functional Receptors

Authors:
  • EBE. A. Barnard
    E. A. Barnard
  • DBD. Beeson
    D. Beeson
  • GBG. Bilbe
    G. Bilbe
  • DBD. A. Brown
    D. A. Brown
  • ACA. Constanti
    A. Constanti
  • KHK. Houamed
    K. Houamed
  • TST. G. Smart
    T. G. Smart
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Abstract
The Xenopus oocyte translation system has been developed for the recognition of receptor messenger-RNA molecules. Observations with the nicotinic acetylcholine, GABA, glycine, glutamate and serotonin receptors show that the mRNAs coding for their subunits can be faithfully translated in the oocyte and the products processed, glycosylated, assembled to a receptor structure, inserted correctly in the cell membrane and organised to form the functional ion channel. The specific regulatory interactions between different sites on one receptor, as exemplified in the GABAA receptor, are established in this process. This system is of value for the detection of mRNAs for receptor gene cloning and for the study of receptor assembly and of the expression of multiple receptor genes. The receptor ion channels formed can also be studied in their own right in a highly accessible and controlled situation.

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