Inhibition of Penaeus monodon densovirus replication in shrimp by double-stranded RNA
- 25 March 2010
- journal article
- research article
- Published by Springer Nature in Archives of Virology
- Vol. 155 (6) , 825-832
Abstract
Stunted shrimp caused by Penaeus monodon densovirus (PmDNV) infection is one of the main problems leading to a significant economic loss in Thailand. To control this pandemic disease, a double-stranded-RNA-mediated virus-specific gene silencing approach was applied to inhibit viral replication. In this study, two dsRNAs corresponding to the non-structural protein (ns1) and the structural protein (vp) genes of PmDNV were synthesized and introduced into shrimp haemolymph prior to viral challenge. After allowing viral replication for two weeks, the suppression effect by each dsRNA was evaluated by semi-quantitative PCR and compared with the control. A reduction of PmDNV in shrimp treated with each dsRNA was observed. In contrast, a high level of viral infection was detected in the control group (NaCl). Based on a limited sample number, we reached the tentative conclusion that virus-specific dsRNA can inhibit PmDNV replication, in which the dsRNA-ns1was more effective than the dsRNA-vp.Keywords
This publication has 25 references indexed in Scilit:
- Inhibition of white spot syndrome virus replication in Penaeus monodon by combined silencing of viral rr2 and shrimp PmRab7Virus Research, 2009
- Therapeutic inhibition of yellow head virus multiplication in infected shrimps by YHV-protease dsRNAAntiviral Research, 2007
- YHV-protease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortalityBiochemical and Biophysical Research Communications, 2006
- Double-Stranded RNA Induces Sequence-Specific Antiviral Silencing in Addition to Nonspecific Immunity in a Marine Shrimp: Convergence of RNAInterference and Innate Immunity in the Invertebrate Antiviral Response?Journal of Virology, 2005
- Silencing of yellow head virus replication in penaeid shrimp cells by dsRNABiochemical and Biophysical Research Communications, 2005
- Interfacial Enzymology of Parvovirus Phospholipases A2Journal of Biological Chemistry, 2004
- RNA interferenceNature, 2002
- Detection of hepatopancreatic parvovirus (HPV) of penaeid shrimp by in situ hybridization at the electron microscope levelDiseases of Aquatic Organisms, 2001
- Statistical correlation between severity of hepatopancreatic parvovirus infection and stunting of farmed black tiger shrimp (Penaeus monodon)Aquaculture, 1999
- Characterization of hepatopancreatic parvo-like virus, a second unusual parvovirus pathogenic for penaeid shrimpsJournal of General Virology, 1995