Visualization of nucleosomes in thin sections by stereo electron microscopy.
Authors:
- A L OlinsAOA L Olins
- D E OlinsDOD E Olins
- H ZentgrafHZH Zentgraf
- W W FrankeWFW W Franke
Open Access
- 1 December 1980
- journal article
- research article
- Published by Rockefeller University Press in Journal of Cell Biology
- Vol. 87 (3) , 833-836
Abstract
Nucleosomes (.apprx. 10 nm diameter) were clearly visualized in thin sections (.apprx. 0.1 .mu.m thick) of isolated chicken erythrocytes. The cells were lysed and fixed in low ionic strength buffers that maintained the chromatin as dispersed filaments and prevented the reformation of supranucleosomal structures. Stereo electron micrographs at high magnification demonstrate the stability of nucleosome structure in the dispersed chromatin state during fixation, dehydration and embedding.Keywords
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