Multiplex PCR Method for Detection of Clostridium difficile tcdA, tcdB , cdtA , and cdtB and Internal In-Frame Deletion of tcdC
Authors:
- 1 December 2011
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 49 (12) , 4299-4300
Abstract
A multiplex PCR method was developed for the detection of Clostridium difficile toxin genes tcdA , tcdB , ctdA , and cdtB and the major in-frame deletion types (18, 39, and 54 bp) of tcdC . The method has high specificity for PCR ribotype 027 and may identify other C . difficile strains of clinical and epidemiological importance.Keywords
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