Interfacing Sca‐1pos Mesenchymal Stem Cells with Biocompatible Scaffolds with Different Chemical Composition and Geometry

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Abstract
An immortalized murine mesenchymal stem cell line (mTERT‐MSC) enriched for Linneg/Sca‐1pos fraction has been obtained through the transfection of MSC with murine TERT and single‐cell isolation. Such cell line maintained the typical MSC self‐renewal capacity and continuously expressed MSC phenotype. Moreover, mTERT‐MSC retained the functional features of freshly isolated MSC in culture without evidence of senescence or spontaneous differentiation events. Thus, mTERT‐MSC have been cultured onto PLA films, 30 and 100 μm PLA microbeads, and onto unpressed and pressed HYAFF‐11 scaffolds. While the cells adhered preserving their morphology on PLA films, clusters of mTERT‐MSC were detected on PLA beads and unpressed fibrous scaffolds. Finally, mTERT‐MSC were not able to colonize the inner layers of pressed HYAFF‐11. Nevertheless, such cell line displayed the ability to preserve Sca‐1 expression and to retain multilineage potential when appropriately stimulated on all the scaffolds tested.
Funding Information
  • Italian Institute for Cardiovascular Research (N40G.25)
  • Istituto Superiore di Sanità (N40G.25)

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