A novel γ subunit of the GABAA receptor identified using the polymerase chain reaction
Authors:
- Misi RobinsonMRMisi Robinson
- Carla GambaranaCGCarla Gambarana
- Ruth E. SiegelRSRuth E. Siegel
- James M. SikelaJSJames M. Sikela
- 24 June 1991
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 284 (2) , 211-215
Abstract
We have utilized a polymerase chain reaction (PCR) strategy to identify a novel subunit, γ3, of the GABAA receptor. The γ3 cDNA encodes a mature protein of 450 amino acids that contains structural features typically conserved among subunits of the GABAA receptor family. The γ3 subunit shares approximately 66% sequence identity with the γ2 subunit but only 38% and 29% with α1 and β1 subunits, respectively. Localization of the γ3 mRNA indicates that it is widely distributed throughout the mouse brain in a pattern similar to that observed for mRNAs encoding the γ2 subunits.Keywords
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