Insulin-binding aptamer-conjugated graphene oxide for insulin detection
- 26 August 2011
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 136 (20) , 4138-4140
Abstract
This paper describes a simple and sensitive aptamer/graphene oxide (GO) based assay for insulin detection. GO can protect DNA from nuclease cleavage, but aptamers can be detached from the GO surface by specific target binding. This exposes the aptamers to enzymatic cleavage and releases the target for a new cycle. Cycling of targets leads to significant signal amplification and low LOD.Keywords
This publication has 20 references indexed in Scilit:
- Type 1 Diabetes: Etiology, Immunology, and Therapeutic StrategiesPhysiological Reviews, 2011
- Amplified Aptamer‐Based Assay through Catalytic Recycling of the AnalyteAngewandte Chemie International Edition, 2010
- Selection of DNA aptamers against insulin and construction of an aptameric enzyme subunit for insulin sensingBiosensors and Bioelectronics, 2009
- Aptamer-Based Au Nanoparticles-Enhanced Surface Plasmon Resonance Detection of Small MoleculesAnalytical Chemistry, 2008
- Regulation of Singlet Oxygen Generation Using Single-Walled Carbon NanotubesJournal of the American Chemical Society, 2008
- Selection‐by‐function: efficient enrichment of cathepsin E inhibitors from a DNA libraryJournal of Molecular Recognition, 2006
- Aptamers evolved from live cells as effective molecular probes for cancer studyProceedings of the National Academy of Sciences, 2006
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990
- Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA PolymeraseScience, 1990
- Radioimmunological determination of human C-peptide in serumDiabetologia, 1975