A Novel Modified Invertase‐Saccharomyces Cerevisiae Hybrid Biosensor for the Determination of Sucrose

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Abstract
A biosensor system based on invertase‐Saccharomyces cerevisiae hybrid system has been developed for the determination of sucrose molecule. For the determination of sucrose in the linear determination range of 0.1–5.0 µM, at 0.5 V potential, the limit of detection (LOD) was found to be 0.08 µM and the limit of quantification (LOQ) was 0.25 µM. With repeatability experiments, the mean value (), standard deviation (±S.D.), and % coefficient of variation (% V.K.) were found to be 1.81 µM, ±0.09 µM, and 4.93%, respectively. Substrate specificity, interference effect, and storage stability were investigated. Sucrose was determined in fruit juice samples.

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