Measurement of regional brain glucose utilization in vivo using [2(-14)C] glucose.
Authors:
- R HawkinsRHR Hawkins
- W K HassWHW K Hass
- J RansohoffJRJ Ransohoff
- 1 November 1979
- journal article
- research article
- Published by Wolters Kluwer Health in Stroke
- Vol. 10 (6) , 690-703
Abstract
A new technique is described for the autoradiographic determination of regional brain glucose metabolism employing 14C labeled glucose as substrate and measurement principles previously described for whole brain. Regional glucose values correlate closely with those reported for the 14C-deoxyglucose technique. The method has the advantages of 1) a much shorter experimental period, 2) a relatively simple mathematical treatment, and 3) the utilization of the actual, fully metabolizable substance itself, glucose, as the label. In addition to normal rats, regional values are reported for 20 individual brain areas of rats in bicuculline induced status epilepticus, rats intoxicated with ammonium and rats anesthetized with pentobarbital sodium or ketamine.Keywords
This publication has 44 references indexed in Scilit:
- Sequential cerebral biochemical and physiological events in controlled hypoxemiaAnnals of Neurology, 1977
- Cytochemical localization of glucose-6-phosphatase activity in the central nervous system of the ratBrain Research, 1976
- A Method for Determining Blood Flow and Oxygen Consumption in the Rat BrainActa Physiologica Scandinavica, 1976
- CEREBRAL ENERGY METABOLISM DURING EXPERIMENTAL STATUS EPILEPTICUS1Journal of Neurochemistry, 1975
- Mapping of Functional Neural Pathways by Autoradiographic Survey of Local Metabolic Rate with [ 14 C]DeoxyglucoseScience, 1975
- CHANGES IN CARBOHYDRATE SUBSTRATES, AMINO ACIDS AND AMMONIA IN THE BRAIN DURING INSULIN‐INDUCED HYPOGLYCEMIAJournal of Neurochemistry, 1974
- Metabolism of oxygen, glucose, lactate and pyruvate in the rat brain in situJournal of Neurochemistry, 1974
- The transport of glucose into the brain of the rat in vivoProceedings of the Royal Society of London. B. Biological Sciences, 1973
- Exchange of Glucose between Plasma, Brain Extracellular Fluid and Cerebral Ventricles in Cats and Effects of Intraventricular Acetazolamide and InsulinActa Physiologica Scandinavica, 1970
- ON THE INTRACELLULAR DISTRIBUTION OF GLUCOSE-6-PHOSPHATASE IN THE NEURONS OF CEREBRUM AND TRIGEMINAL GANGLION OF THE RATJournal of Histochemistry & Cytochemistry, 1963