Induction of Various HLA Class II Molecules in a Human Colonic Adenocarcinoma Cell Line

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Abstract
The colonic carcinoma cell line HT‐29 had no constitutive expression of HLA class II molecules. Gamma interferon (IFN‐γ) induced expression of HLA class II molecules in a dosedependent manner with 100 U/ml as an optimal dose. The expression of HLA‐DR, HLA‐DP, and HLA‐DQ molecules seemed to follow different kinetics. While DR and DP molecules were maximally induced after 2 days, DQ molecules appeared later with maximum percentage positive cells after 8 days. Treatment with a prostaglandin synthesis inhibitor (indomethacin) neither induced class II expression nor altered the dose‐response curve for IFN‐γ; this indicated that possible endogenous production of prostaglandins iin this cell line did not interfere with its class II expression. The lectins phytohaemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), and wheat germ agglutinin (WGA) did not induce class II expression.

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