A Nickel Superoxide Dismutase Maquette That Reproduces the Spectroscopic and Functional Properties of the Metalloenzyme
Authors:
- 11 February 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Inorganic Chemistry
- Vol. 45 (6) , 2358-2360
Abstract
Described herein is a nickel superoxide dismutase (NiSOD) maquette ([Ni(SODM1)]) based on the first 12 residues from the N-terminal sequence of Streptomycescoelicolor NiSOD. The apopeptide (SODM1) was prepared by standard solid-phase Fmoc peptide synthesis. SODM1 will readily coordinate NiII in a 1:1 ratio in slightly basic aqueous sodium phosphate buffer (0.1 M; pH = 7.2) forming a lightly colored beige/pink solution. Unlike NiSOD, which is isolated as a 1:1 mixture of oxidized (NiIII) and reduced (NiII) forms, [Ni(SODM1)] can only be isolated in the NiII oxidation state. The UV/vis, X-ray absorption, and CD spectra of [NiII(SODM1)] correspond well with those reported for the reduced form of NiSOD. Despite the fact that [NiIII(SODM1)] is not isolable, [Ni(SODM1)] has an appropriate redox potential to act as an SOD (E1/2 = 0.70(2) V vs Ag/AgCl) and in fact will catalytically disproportionate >40 000 equiv of KO2.Keywords
This publication has 11 references indexed in Scilit:
- Investigating Metalloenzyme Reactions Using Electrochemical Sweeps and Steps: Fine Control and Measurements with Reactants Ranging from Ions to GasesInorganic Chemistry, 2005
- Crystal structure of nickel-containing superoxide dismutase reveals another type of active siteProceedings of the National Academy of Sciences, 2004
- Nickel Superoxide Dismutase Structure and MechanismBiochemistry, 2004
- The genome of a motile marine SynechococcusNature, 2003
- Enzyme Electrokinetics: Using Protein Film Voltammetry To Investigate Redox Enzymes and Their MechanismsBiochemistry, 2003
- Examination of the Nickel Site Structure and Reaction Mechanism in Streptomyces seoulensis Superoxide DismutaseBiochemistry, 1999
- Oxygen Capture by Sulfur in Nickel ThiolatesAccounts of Chemical Research, 1998
- Enzyme Bioelectrochemistry in Cast Biomembrane-Like FilmsAccounts of Chemical Research, 1998
- Toward the Synthesis of a Photosynthetic Reaction Center Maquette: A Cofacial Porphyrin Pair Assembled between Two Subunits of a Synthetic Four-Helix Bundle Multiheme ProteinJournal of the American Chemical Society, 1996
- Metalloenzymes, Structural Motifs, and Inorganic ModelsScience, 1993