Noninvasive In Vivo Monitoring of Extracellular Vesicles
Authors:
- 4 October 2013
- book chapter
- Published by Springer Nature
- Vol. 1098, 249-258
Abstract
Extracellular vesicles (EVs) including exosomes and microvesicles are nanometer-sized vesicles released by cells to deliver lipids, cellular proteins, mRNAs, and noncoding RNAs, thereby facilitating intercellular communication without direct cell-to-cell contacts. Due to their nanoscale size, EVs have been visualized under microscopy in vitro. We here describe a strategy to label EVs with Gaussia luciferase for noninvasive bioluminescence imaging and monitoring of systemically administered EVs in vivo.Keywords
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