Solvatochromic Two-Photon Fluorescent Probe Enables In Situ Lipid Droplet Multidynamics Tracking for Nonalcoholic Fatty Liver and Inflammation Diagnoses

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Abstract
Intracellular lipid storage and regulation occur in lipid droplets, which are of great significance to the physiological activities of cells. Herein, a lipid droplet-specific fluorescence probe (lip-YB) with a high quantum yield (QYlip-YB = 73.28%), excellent photostability, and quickly polarity sensitivity was constructed successfully. Interestingly, lip-YB exhibited remarkable two-photon (TP) characteristics, which first realized real-time monitoring of the lipid droplet multidynamics process, diagnosing nonalcoholic fatty liver disease (NAFLD) and inflammation in living mice via TP fluorescence imaging. It is found that the as-prepared lip-YB provides a new avenue to design lipid droplet-specific imaging probes, clarifies its roles and mechanisms in cell metabolism, and can timely intervene in lipid droplet-related diseases during various physiological and pathological processes.
Funding Information
  • Ministry of Education of the People's Republic of China
  • Anhui University (S020118002/039, S020118002/089, S020318006/005)
  • National Natural Science Foundation of China (22005001, 22005002, 22005005, 51972001)
  • Academician Workstation, Anhui University (PECL2019KF012)
  • Key Lab of Photovoltaic and Energy Conservation Materials, Chinese Academy of Sciences

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