TheSambucus nigratype‐2 ribosome‐inactivating protein SNA‐I′ exhibits in planta antiviral activity in transgenic tobacco
Authors:
- Willy J PeumansWPWilly J Peumans
- Els J.M Van DammeEls J.M Van Damme
- 8 March 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 516 (1-3) , 27-30
Abstract
Transgenic tobacco (Samsun NN) plants transformed with a cDNA clone encoding SNA‐I′ fromSambucus nigrasynthesize, and correctly process and assemble, a fully active type‐2 ribosome‐inactivating protein. Expression of SNA‐I′ under the control of the 35S cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. In contrast to type‐1 ribosome‐inactivating proteins, the expression of SNA‐I′ does not affect the growth and fertility of the transgenic plants and is not accompanied by an increased expression of pathogenesis‐related proteins indicating that its antiviral activity most probably differs from that of pokeweed antiviral protein.Keywords
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