Time resolved EPR on photosystem II particles after irreversible and reversible inhibition of water cleavage with high concentrations of acetate

Authors:
  • CBChristian H. Bock
    Christian H. Bock
  • SGStephan Gerken
    Stephan Gerken
  • DSDietmar Stehlik
    Dietmar Stehlik
  • HWHorst T. Witt
    Horst T. Witt

Abstract
Electron transfer in irreversibly acetate‐inhibited PS II preparations fromSynechococcusis investigated with EPR. A 1.4 mT wide EPR line with a decay rate consistent with the P+680Q−Aback reaction is tentatively assigned to be a broadened P+680spectrum. With reversible inhibition of water cleavage by the ADRY reagent FCCP added with acetate electron transfer is studied as well. Under these conditions the spectrum of the immediate donor to P+680is the same as the spectrum of Z+in systems with irreversibly inhibited water cleavage (signal II fast) and the same as that of D+(signal II slow). The consequences are discussed.

This publication has 22 references indexed in Scilit: