METHOD FOR DETERMINING THE α‐TOCOPHEROL CONTENT OF CITRUS ESSENTIAL OILS
Authors:
- 1 March 1976
- journal article
- research article
- Published by Wiley in Journal of Food Science
- Vol. 41 (2) , 370-371
Abstract
A simple and reliable method for determining the amount of naturally occurring α‐tocopherol in citrus essential oils is presented. The unsaponified oil is streaked directly onto a Silica Gel G TLC plate and developed in a benzene‐methanol solution to effect separation of tocopherols. The α‐tocopherol band is scraped from the plate and eluted with small volumes of absolute ethanol so that subsequent concentration of the fraction is not necessary. Ferric chloride‐bipyridyl reagents are added to the eluent and the absorption at 520 nm is determined using a spectrophotometer. A standard curve is prepared using the same procedure so as to eliminate the need for using correction factors.Keywords
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