Lymphocyte Subpopulations in Man: No Selective Loss during Isolation from Defibrinated Blood

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Abstract
Human lymphocytes isolated from defibrinated blood are characterized especially in relation to the total yield of lymphocytes and their subpopulations identified by membrane markers. The defibrination per se gives a significant loss of monocytes and granulocytes but no loss of lymphocytes, indicating that no selective loss of lymphocytes occurs. In a comparison of heparin-stabilized and defibrinated blood no difference in yield is found during every single step of the isolation procedure. Quantitation of E-RFC [erythrocyte rosette forming cell] and SmIg[surface membrane immunoglobulin]-positive lymphocytes gives no differences in comparing the respective isolated suspensions of mononuclear cells. The observed difference in EA[erythrocyte, antibody]- and EAC[erythrocyte, antibody, complement]-RFC can freely be ascribed to the difference in monocyte contamination. No correlation is found between total lymphocyte yield and the relative number of the subpopulations identified, i.e., E-RFC, SmIg-positive, Fc-receptor- and complement-receptor-bearing lymphocytes. Hence defibrinated blood is optimal as blood source for the isolation of mononuclear cells when lymphocyte subpopulations are studied and enumerated.

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