Chemical Composition of Basophil Granules from Isolated Rat Mast Cells
Authors:
- 1 November 1971
- journal article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 83 (3) , 362-372
Abstract
A protein fraction was isolated from rat mast cell granules. Disc gel electrophoresis and peptide mapping showed this fraction to be a mixture of 1 or 2 main components and 2–3 minor components. On ultracentrifugation the protein fraction behaved as an homogeneous material with a molecular weight of 5600. Amino acid analysis showed the presence of all the common amino acids (with the possible exception of tryptophan). High contents of lysine, aspartic acid, glutamic acid, proline, glycine and valine were noted. The content of cysteine was remarkably low. The isoelectric point of the main component(s) was found to be around 9.Keywords
This publication has 32 references indexed in Scilit:
- N-Acetyl-β-glucosaminidase in rat mast cell granulesExperimental Cell Research, 1970
- Selective incorporation of zinc into rat mast cellsCellular and Molecular Life Sciences, 1970
- Free Zone Electrophoresis. Theory, Equipment, and Applications1970
- The morphology of the heparin-protein macromolecule and its organization in the mast cell granuleJournal of Ultrastructure Research, 1969
- THE ZINC CONTENT OF RAT MAST CELLSImmunology & Cell Biology, 1968
- The Amino Acid Sequence of Oxidized Viscotoxin A3 from the European Mistletoe (Viscum album L, Loranthaceae).Acta Chemica Scandinavica, 1968
- The Efects of Compound 48/80 and Distilled Water on the Adenosine Triphosphate Content of Isolate Rat Mast CellsActa Physiologica Scandinavica, 1967
- Ionic Binding of Histamine in Mast Cell GranulesActa Physiologica Scandinavica, 1967
- Mode of Storage of Histamine in Mast CellsActa Physiologica Scandinavica, 1966
- Bedeutung von Zink für die Histaminspeicherung in MastzellenInternational Archives of Allergy and Immunology, 1963