The untranslated leader sequence of the barleylipoxygenase 1(Lox1) gene confers embryo‐specific expression

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Summary: The barley lipoxygenase 1 ( Lox1 ) gene encodes a protein expressed in embryos during grain development and germination and in leaves after methyl‐jasmonate (MeJA) treatment. Transient gene expression assays in germinating barley embryos were used to identify cis ‐regulatory elements involved in the embryo‐specific expression of the Lox1 gene. Analysis of transcriptional or translational fusions between Lox1 5′ upstream sequences and the gusA reporter gene indicated that the 5′‐untranslated leader sequence was involved in embryo‐specific expression. Replacement of the leader sequence from the aleurone‐specific Chi26 gene with the Lox1 leader sequence resulted in a chimeric gene expressed at high levels in embryo as well as in aleurone cells. Insertion of the Lox1 leader sequence between the 35S minimum promoter (A domain –90/+8) and the gusA reporter gene greatly enhanced promoter activity in a tissue‐specific manner. Deletion/replacement analysis of the Lox1 leader sequence, combined with transient expression in germinating embryos and in vitro transcription/translation assays, suggests that the Lox1 leader sequence contains cis ‐elements regulating qualitative (tissue‐specific) and quantitative gene expression.

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