Correction of light‐scattering errors in spectrophotometric protein determinations
Authors:
- 1 July 1971
- journal article
- research article
- Published by Wiley in Biopolymers
- Vol. 10 (7) , 1243-1251
Abstract
Errors due to light scattering are a feature of spectrophotometrie methods of protein determination. The experimental conditions may be chosen to allow calculation of the scatter contribution, which is obtained essentially from the linear relationship between log turbidity and log incident wavelength, together with knowledge of the slope of such functions. Using this information, the scatter corrections may be obtained by various simple and rapid methods, applicable to single or multicomponent protein solutions.Keywords
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