Polewards chromosome movement driven by microtubule depolymerization in vitro
Authors:
- T. J. MitchisonTMT. J. Mitchison
- Marc W. KirschnerMKMarc W. Kirschner
- 1 February 1988
- journal article
- Published by Springer Nature in Nature
- Vol. 331 (6156) , 499-504
Abstract
We constructed complexes between isolated chromosomes and microtubules made from purified tubulin to study the movement of chromosomes towards the 'minus' end of microtubules in vitro, a process analogous to the movement of chromosomes towards the pole of the spindle at anaphase of mitosis. Our results show that the energy for this movement is derived solely from microtubule depolymerization, and indicate that anaphase movement of chromosomes is both powered and regulated by microtubule depolymerization at the kinetochore.Keywords
This publication has 28 references indexed in Scilit:
- On the mechanism of anaphase A: evidence that ATP is needed for microtubule disassembly and not generation of polewards force.Journal of Cell Biology, 1987
- Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.Journal of Cell Biology, 1987
- Beyond self-assembly: From microtubules to morphogenesisCell, 1986
- Properties of the kinetochore in vitro. I. Microtubule nucleation and tubulin binding.Journal of Cell Biology, 1985
- Unstained microtubules studied by cryo-electron microscopyJournal of Molecular Biology, 1985
- Structural polarity of kinetochore microtubules in PtK1 cells.Journal of Cell Biology, 1981
- Chromosome behavior after laser microirradiation of a single kinetochore in mitotic PtK2 cells.Journal of Cell Biology, 1981
- Cell division and the mitotic spindle.Journal of Cell Biology, 1981
- Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus).Journal of Cell Biology, 1980
- A new cleavable reagent for cross-linking and reversible immobilization of proteinsBiochemical and Biophysical Research Communications, 1979