Transient expression of Arabidopsis thaliana ascorbate peroxidase 3 in Nicotiana benthamiana plants infected with recombinant potato virus X
- 21 February 2003
- journal article
- biotic and-abiotic-stress
- Published by Springer Nature in Plant Cell Reports
- Vol. 21 (7) , 699-704
Abstract
We have explored the transient over-expression of Arabidopsis thaliana ascorbate peroxidase 3 (APX3) in Nicotiana benthamiana using a viral vector based on the potato virus X (PVX). Plants infected with a PVX:APX3 hybrid had a similar progression of viral particles compared to control plants infected with a PVX:GFP hybrid, indicating that infection was not affected by the over-expression of heterologous APX3. Our results also showed that in PVX:APX3-infected plants, the hybrid virus directed a high level of APX3 expression and the recombinant protein was functional, as inferred from the higher APX activity compared to mock and PVX:GFP hybrid-infected plants. The PVX recombinant expression system used is a simple and quick method for transient expression of heterologous APXs, which are expected to suffer specific processing in plant cells.Keywords
This publication has 29 references indexed in Scilit:
- Production of Antimicrobial Defensin in Nicotiana benthamiana with a Potato Virus X VectorMolecular Plant-Microbe Interactions®, 2001
- Peroxisomal Membrane Ascorbate Peroxidase Is Sorted to a Membranous Network That Resembles a Subdomain of the Endoplasmic ReticulumThe Plant Cell, 1999
- Role of the Ascorbate-Glutathione Cycle of Mitochondria and Peroxisomes in the Senescence of Pea LeavesPlant Physiology, 1998
- Post-Transcriptional Suppression of Cytosolic Ascorbate Peroxidase Expression during Pathogen-Induced Programmed Cell Death in TobaccoThe Plant Cell, 1998
- Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress.The Plant Cell, 1997
- Ascorbate Peroxidase (A Prominent Membrane Protein in Oilseed Glyoxysomes)Plant Physiology, 1996
- Jellyfish green fluorescent protein as a reporter for virus infectionsThe Plant Journal, 1995
- Potato virus X as a vector for gene expression in plantsThe Plant Journal, 1992
- Purification and Characterization of Pea Cytosolic Ascorbate PeroxidasePlant Physiology, 1991
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976