Supplementary Figure S5 from Differential Effects of High-Fiber and Low-Fiber Diets on Antitumor Immunity and Colon Tumor Progression in a Murine Model

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Abstract
Supplementary Figure S5 shows the growth response of ETBF to a high-fiber diet in APCmin/+ mice. Mice were inoculated with ETBF at day 0 and fed either a low-fiber or high-fiber diet. Fecal samples were collected at designated time points, and the expression of B. fragilis toxin 2 (BFT-2) was measured using qPCR as a surrogate for ETBF abundance. The relative percentage of ETBF expression at each time point was calculated relative to pre-inoculation levels.