High-Level Penicillin Resistance among Isolates of Enterococci

Authors:
  • JCJulieta Calmon
    Julieta Calmon
  • CCClare L. Cherney
    Clare L. Cherney
  • MWMichael Wendeler
    Michael Wendeler
  • PPPeter Pitsakis
    Peter Pitsakis
  • JPJames Poupard
    James Poupard
  • MLMatthew E. Levison
    Matthew E. Levison
  • CJCaroline C. Johnson
    Caroline C. Johnson
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Abstract
To determine the extent and clinical significance of high-level penicillin-resistant Enterococcus faecium at our institution. Surveillance of clinical enterococcal isolates, in-vitro susceptibility and timed survival studies, and determination of antibiotic efficacy in an experimental model of enterococcal endocarditis. For a 6-month period, 14% of enterococcal isolates (30 of 212) were identified as E. faecium. One third of the isolates were highly resistant to penicillin G (minimum inhibitory concentration [MIC], greater than or equal to 200 micrograms/mL) but did not produce beta-lactamase. The findings from in-vitro survival studies showed that this high-level resistance resulted in the loss of bactericidal activity normally observed when an aminoglycoside antibiotic agent is combined with penicillin. An experimental rat model of endocarditis provided in-vivo data that confirmed our in-vitro observations. After the rats received therapy for 72 hours, penicillin G either alone or in combination with gentamicin did not significantly decrease the numbers of enterococci in vegetations on heart valves compared with untreated controls (P = 0.62 and P = 0.58, respectively). Enterococcus faecium accounts for a notable proportion of clinical enterococcal isolates. Many strains from patients at our institution, as well as from patients at other institutions throughout the country, are highly resistant to penicillin. Because high-level penicillin resistance has important therapeutic implications, periodic surveillance and MIC testing of significant enterococcal isolates, especially E. faecium, are suggested.

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