High-resolution episcopic microscopy (HREM) is a novel method producing high-quality, three-dimensional (3D) images of tissue from a variety of species. We report, for the first time, the use of HREM to create high-resolution 3D reconstructions of the human foetal heart in the first trimester. These models are suitable for a detailed morphological investigation to produce ‘virtual’ images of any section plane and 3D models that can be examined using ‘virtual’ dissection.