Isolation and characterization of a spacerless dinucleosome from Hl-depleted chromatin
Authors:
- Leonard KlevanLKLeonard Klevan
- Donald M. CrothersDCDonald M. Crothers
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (12) , 4077-4090
Abstract
Calf thymus chromatin, depleted in histone H1, was digested with micrococcal nuclease and fractionated by column chromatography. 140 base pair nucleosome core particles were isolated along with an unusual particle containing 2 histone octamers and 240 base pairs of DNA. Evidence is presented that the spacer DNA region is absent from these modified dinucleosomes, which appear as stable products of the digestion process. The physical properties of both particles are presented along with brief speculation on their possible origin and function.Keywords
This publication has 34 references indexed in Scilit:
- Quantitative analysis of the digestion of yeast chromatin by staphylococcal nucleaseBiochemistry, 1977
- Comparative subunit structure of HeLa, yeast, and chicken erythrocyte chromatin.Proceedings of the National Academy of Sciences, 1977
- Action of micrococcal nuclease on chromatin and the location of histone H1Journal of Molecular Biology, 1977
- Nuclease digestion of reconstituted chromatinFEBS Letters, 1976
- Subunit structure of rDNA-containing chromatinBiochemistry, 1976
- The isolation of nucleosomes from saline‐washed chromatinFEBS Letters, 1976
- Thermal denaturation of subchromosomal particlesBiochemical and Biophysical Research Communications, 1975
- Chromatin Fragments Resembling ν BodiesScience, 1975
- Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonucleaseBiochemical and Biophysical Research Communications, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970