Male‐to‐male differences in post‐thaw motility of rhesus spermatozoa after cryopreservation of replicate ejaculates

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Abstract
Background The efficiency of controlled propagation to produce rhesus monkeys of particular genotypes can be maximized by use of cryopreserved spermatozoa collected from specific males to inseminate appropriate females. But this assumes that semen from males with different genotypes can be cryopreserved with equal effectiveness.Methods To investigate whether spermatozoa from differentMacaca mulattamales can be effectively cryopreserved when frozen under identical conditions, we collected and froze semen specimens from 13 adult, fertile males maintained at three primate research centers.Results Survival, based on post‐thaw motility normalized to the pre‐freeze value, was assayed within 30 minutes after thawing; it varied from 50% to 70% but declined thereafter. To examine the response of semen from individual males, we collected and froze three to six ejaculates per male from each of seven males.Conclusions In general, semen from a given male responded reproducibly to freezing, but there were significant differences among males. The cause of these differences amongM. mulattamales in post‐thaw sperm survival remains unidentified.

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