Fertilization of Steelhead Trout (Salmo gairdneri) Eggs With Cryo-preserved Sperm

Authors:
  • AOA. G. Ott
    A. G. Ott
  • HHH. F. Horton
    H. F. Horton
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Abstract
The average percent fertilization of fresh steelhead trout (Salmo gairdneri) ova inseminated with cryo-preserved sperm was 59 in 90 tests. Sodium bicarbonate, lecithin, and mannitol at 750, 750, and 250 mg/100 ml, respectively, were included in the extender. The most effective concentration of dimethyl sulfoxide in the extender was 12% (v/v). Significant differences in percent of fresh ova fertilized with cryo-preserved sperm were attributed to both parents.