Rapid Screening of Topoisomerase Gene Mutations by a Novel Melting Curve Analysis Method for Early Warning of Fluoroquinolone-Resistant Streptococcus pneumoniae Emergence
Authors:
- 1 December 2006
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (12) , 4553-4558
Abstract
We developed a real-time PCR assay combined with melting curve analysis for rapidly genotyping quinolone resistance-determining regions (QRDR) of topoisomerase genes in Streptococcus pneumoniae . This assay was not only accurate for the screening of fluoroquinolone (FQ) resistance but also relevant as an early warning system for detecting preexisting single QRDR mutations.Keywords
This publication has 29 references indexed in Scilit:
- Rapid Screening of Fluoroquinolone Resistance Determinants in Streptococcus pneumoniae by PCR-Restriction Fragment Length Polymorphism and Single-Strand Conformational PolymorphismJournal of Clinical Microbiology, 2006
- Rapid detection of a chromosomally mediated penicillin resistance-associatedponAmutation inNeisseria gonorrhoeaeusing a real-time PCR assayFEMS Microbiology Letters, 2006
- Real-Time PCR Screening for 16S rRNA Mutations Associated with Resistance to Tetracycline in Helicobacter pyloriAntimicrobial Agents and Chemotherapy, 2005
- Investigation of the susceptibility trends in Japan to fluoroquinolones and other antimicrobial agents in a nationwide collection of clinical isolates: a longitudinal analysis from 1994 to 2002Diagnostic Microbiology and Infectious Disease, 2005
- Quinolone Resistance Mechanisms in PneumococciClinical Infectious Diseases, 2004
- Quinolone Resistance Mechanisms in PneumococciClinical Infectious Diseases, 2004
- An extended PCR-RFLP assay for detection of parC, parE and gyrA mutations in fluoroquinolone-resistant Streptococcus pneumoniaeJournal of Antimicrobial Chemotherapy, 2004
- PCR-Oligonucleotide Ligation Assay for Detection of Point Mutations Associated with Quinolone Resistance in Streptococcus pneumoniaeAntimicrobial Agents and Chemotherapy, 2003
- Fluoroquinolone-Resistant Streptococcus pneumoniae Strains Occur Frequently in Elderly Patients in JapanAntimicrobial Agents and Chemotherapy, 2002
- Mechanisms of fluoroquinolone resistanceDrug Resistance Updates, 1999