Annexins V and VI: major calcium-dependent atrial secretory granule-binding proteins.

Authors:
  • CKChantal Kang
    Chantal Kang
  • MDMiguel Dominguez
    Miguel Dominguez
  • SLStéphane Loyau
    Stéphane Loyau
  • TMToshiyuki Miyata
    Toshiyuki Miyata
  • VDVincent Durlach
    Vincent Durlach
  • EAEdouard Anglés-Cano
    Edouard Anglés-Cano
  • BPBehnaz Parhami-Seren
    Behnaz Parhami-Seren
  • RHRichard Haberly
    Richard Haberly
  • MMMichael N. Margolies
    Michael N. Margolies
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Abstract
Atrial natriuretic peptide is stored by atrial myocytes in secretory granules, known as atrial specific granules, and is released from these granules by exocytosis. We have isolated a group of atrial proteins by affinity chromatography that bind to atrial specific granules in a calcium-dependent manner. The two major proteins isolated (32.5 kd and 67 kd) are calcium-binding proteins and have been identified as annexins V and VI by immunoblotting with specific antisera. The calcium dependence of their binding to atrial specific granules has been characterized in vitro and indicates that this interaction takes place at micromolar levels of calcium. In addition, the group of proteins isolated includes another calcium-binding protein of 20 kd, as well as GTP-binding proteins of 22 to 26 kd. Membrane interactions during exocytosis are presumably mediated by the interaction of specific proteins with the granule membrane. The properties of the proteins described here, and their ability to bind to atrial specific granules in a calcium-dependent manner, make them likely candidates in the search for regulatory proteins mediating atrial natriuretic peptide secretion.

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