A Simple Immunoassay for Insulin: Application to Human and Dog Plasma

Authors:
  • BGBeryl Z. Greenberg
    Beryl Z. Greenberg
  • JEJoyce Ells
    Joyce Ells
  • CMCurtis Meinert
    Curtis Meinert
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Abstract
A rapid and convenient radio immunoassay for insulin is presented, using iodine131-labeled insulin of intermediate specific activity (20–30 mc/mg). The method is based on the in vitro system of Skom and Talmage for detection of insulin antibody, and involves precipitation of insulin-antibody complex by a second antibody against gamma globulin. The use of moderately labeled insulin is made possible by the relatively large volume of plasma conveniently handled in a test tube system. Application of the method to human and dog plasma is described.

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