Protective Effect of Vegan Microbiota on Liver Steatosis Is Conveyed by Dietary Fiber: Implications for Fecal Microbiota Transfer Therapy
Authors:
- Nikola DaskovaNDNikola Daskova
- Marie HeczkovaMHMarie Heczkova
- Istvan ModosIMIstvan Modos
- Jaromir HradeckyJHJaromir Hradecky
- Tomas HudcovicTHTomas Hudcovic
- Marek KuzmaMKMarek Kuzma
- Helena PelantovaHPHelena Pelantova
- Irena BuskovaIBIrena Buskova
- Eva SticovaESEva Sticova
- David FundaDavid Funda
- Jaroslav GoliasJGJaroslav Golias
- Barbora DrabonovaBDBarbora Drabonova
Open Access
Abstract
Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics. We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects. Ex-germ-free animals were colonized with human vegan microbiota and fed a standard or Western-type diet (WD) with or without inulin supplementation. Despite the colonization with vegan microbiota, WD induced excessive weight gain, impaired glucose metabolism, insulin resistance, and liver steatosis. However, supplementation with inulin reversed steatosis and improved glucose homeostasis. In contrast, inulin did not affect WD-induced metabolic changes in non-humanized conventional mice. In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA). We found that (i) vegan microbiota alone does not protect against adverse effects of WD; and (ii) supplementation with inulin reversed steatosis and normalized glucose metabolism. This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.Keywords
Funding Information
- Ministry of Health of the Czech Republic (NV-18-01-00040, IN 00023001, LX22NPO5104, LX22NPO5104)
- MH CR–DRO (Institute for Clinical and Experimental medicine–IKEM (NV-18-01-00040, IN 00023001, LX22NPO5104, LX22NPO5104)
- project National Institute for Research of Metabolic and Cardiovascular Diseases (NV-18-01-00040, IN 00023001, LX22NPO5104, LX22NPO5104)
- European Union—Next Generation EU (NV-18-01-00040, IN 00023001, LX22NPO5104, LX22NPO5104)
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