An Inhibitor of Endopeptidase‐24.15 Blocks the Degradation of Intraventricularly Administered Dynorphins

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Abstract
Conversion of the octapeptide dynorphin (Dyn) A‐(1–8) to Leu5‐enkephalin (LE) by endopeptidase EC 3.4.24.15 (EP‐24,15) in vivo was examined using the technique of ventriculocisternal perfusion. Peptides were administered intra‐cerebroventricularly in the presence or absence of the EP‐24.15 inhibitor N‐[1‐(R,S)‐carboxy‐3‐phenylpropyl]‐Ala‐Ala‐Phe‐p‐aminobenzoate (cFPAAF‐pAB) via cannulae placed into the lateral ventricle of urethane‐anesthetized rats. The concentration of Dyn‐like peptides and LE within the CSF was monitored by radioimmunoassay in samples of CSF taken from a second cannula placed in the cisterna magna. In the absence of inhibitor, Molíneaux C. J. and Ayala J. M. An inhibitor of endopeptidase‐24.15 blocks the degradation of intraventricularly administered dynorphins. J. Neurochem. 55, 611–618 (1990).

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