Production of a variant of β-lactamase II with selectively decreased cephalosporinase activity by a mutant of Bacillus cereus 569/H/9

Authors:
  • GEG F S Edwards
    G F S Edwards
  • PKP A Kiener
    P A Kiener
  • MTM J Tully
    M J Tully
  • SWS G Waley
    S G Waley
  • EAE P Abraham
    E P Abraham
Publisher website
Google Scholar
Abstract
Mutants of B. cereus 569/H/9 were screened in a search for strains that synthesize variants of .beta.-lactamase II. One of these mutants (strain 569/H/9/1) produces a .beta.-lactamase II-like enzyme that shows a selective decrease in cephalosporinase activity. .beta.-Lactamase II from strain 569/H/9/1 was purified to apparent homogeneity and its kinetic properties were examined. This enzyme resembles the parent .beta.-lactamase II in its relative activity with benzylpenicillin as substrate when Zn(II) is replaced by other metal ions, but differs detectably from the parent enzyme in its isoelectric point.

This publication has 18 references indexed in Scilit: