pparβ regulates lipid catabolism by mediating acox and cpt-1 genes in Scophthalmus maximus under heat stress

Authors:
Publisher website
Google Scholar
Abstract
Peroxisome proliferator–activated receptor β (pparβ) is a key gene–regulating lipid metabolism pathway, but its function in turbot remains unclear. In this study, the CDS of pparβ was cloned from kidney for the first time. The CDS sequence length was 1533 bp encoding 510 amino acids. Structural analysis showed that the pparβ protein contained a C4 zinc finger and HOLI domain, suggesting that the pparβ gene of turbot has high homology with the PPAR gene of other species. The high expression patterns of pparβ, acox, and cpt-1 at high temperatures, as shown through qPCR, indicated that high temperatures activated the transcriptional activity of pparβ and increased the activity of the acox and cpt-1 genes. The expression of acox and cpt-1 was significantly inhibited when pparβ was downregulated using RNAi technology and inhibitor treatments, suggesting that pparβ positively regulated acox and cpt-1 expression at high temperatures and, thus, modulates lipid catabolism activity. These results demonstrate that pparβ is involved in the regulation of lipid metabolism at high temperatures and expand a new perspective for studying the regulation of lipid metabolism in stress environments of teleost.
Funding Information
  • China Agriculture Research System of MOF and MARA (CARS-47-G01)
  • Agricultural Fine Breed Project of Shandong (2019 LZGC013)
  • Central Public-interest Scientific Institution Basal Research Fund, CAFS (No. 2020TD25)
  • Central Public-interest Scientific Institution Basal Research Fund, YSFRI (No.20603022021009)
  • National Nature Science Foundation of China (No. 32002362)
  • Shandong Provincial Natural Science Foundation, China (No. ZR2019BC089)

This publication has 38 references indexed in Scilit: