Mechanical unfolding of a2‐macroglobulin molecules with atomic force microscope
Authors:
- Masahiko HaraMHMasahiko Hara
- Atsushi IkaiAIAtsushi Ikai
- 29 April 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 385 (1-2) , 29-33
Abstract
α 2‐Macroglobutin was derivatized with a sulfhydryl cross‐linker and sandwiched between a mica substrate and a solicon nitride tip, both coated with gold, of an atomic force microscope and force curve measurement was carried out. An extensive downward deflection of the cantilever was observed in the retracting realm of the curve, when and only when the substrate was covered with the derivatized protein. The result was interpreted in terms of the mechanical stretching and unfolding of a single or a few protein molecules.Keywords
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