Characterization of the OCT plasmid encoding alkane oxidation and mercury resistance in Pseudomonas putida
Authors:
- P A HarderPHP A Harder
- D A KunzDKD A Kunz
Open Access
- 1 February 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 165 (2) , 650-653
Abstract
Transformation of Pseudomonas putida and analysis for plasmid DNA revealed that both n-alkane oxidation and mercury resistance are encoded on a single 220-megadalton OCT plasmid molecule. Derivatives of OCT having lost the mercury resistance function could be readily isolated and contained a smaller plasmid estimated to be 170 megadaltons. The results show that segregation of the mercury resistance property occurs not by loss of a separate MER plasmid as previously thought but by a deletion in the OCT plasmid.Keywords
This publication has 23 references indexed in Scilit:
- IncP2 group of Pseudomonas, a class of uniquely large plasmidsNature, 1978
- Isolation of Inc P-2 plasmid DNA from Pseudomonas aeruginosaPlasmid, 1978
- Molecular characterization of hydrocarbon degradative plasmids in Pseudomonas putidaBiochemical and Biophysical Research Communications, 1977
- Isolation of covalently closed circular DNA of high molecular weight from bacteriaAnalytical Biochemistry, 1976
- PLASMIDS IN PSEUDOMONASAnnual Review of Genetics, 1976
- Genetic Regulation of Octane Dissimilation Plasmid in PseudomonasProceedings of the National Academy of Sciences, 1973
- Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNAProceedings of the National Academy of Sciences, 1972
- Molecular weights of coliphages and coliphage DNAJournal of Molecular Biology, 1970
- Hydrocarbon oxidation by a bacterial enzyme systemBiochimica et Biophysica Acta, 1963
- Transduction of linked genetic characters of the host by bacteriophage P1Virology, 1955